This database allows the comparison of mip gene DNA sequences generated in your laboratory with reference sequences from all described species of Legionella. This sequence-based classification scheme [external link] was first described by Dr. Rod Ratcliff (email@example.com), Infectious Disease Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia, 5000. < http://www.imvs.sa.gov.au/ [external link ]>.
Using the following protocol:
Sequence-based identification of Legionella using mip (PDF, 130 KB)
you will obtain DNA sequence from your Legionella species. isolate. Submission of your sequence will return details of the closest matches within the database entries.You do not require a password to access this databases .
An experimental program has been developed to allow Legionella species to be identified using mip DNA sequence, but with additional features. These include the ability to view an alignment of your sequence with reference sequences and a neighbour-joining phylogenetic tree based on this alignment. If you would like to use this program, please bear in mind that it is still under development and that any feedback via e-mail would be very welcome.
The mip-based Legionella species identification program allows the user to view:
(i) an alignment of the user sequence with reference sequences
(ii) a neighbour-joining phylogenetic tree based on this alignment and
(iii) a small alignment of sequences most similar to the user sequence together with the percentage similarities of the closet matches
Further help with this option [external link]
Last reviewed: 7 January 2013